Thermofluor can detect multiple binding events and cooperativity in protein ligand binding interactions.
Thermofluor can be an efficient means to discriminate the ability of ligands to induce additional binding events (e.g. for nuclear hormone receptor interactions).
Structural determination of estrogen-related receptor gamma in the presence of phenol derivative compounds. Abad MC, et.al. Steroid Biochem Mol Biol. 2008 Jan;108(1-2):44-54. [X-ray crystal structures of NHR ligands found using thermofluor screening] PMID: 17964775