Thermofluor was the major high-througput screening approach used at 3DP from 1999 to 2003. During that time, desire for increased throughput motivated development of a new generation instrument that used a custom 1536-well plate and incorporated an enhanced imaging system allowing reduction in the amount of protein required for a single Tm determination by a factor of 5-10 fold.
A prototype 1536 instrument was built and successfully tested, but development ceased in 2003.
3DP developed a custom 1536-well plate manufactured from a silicon-nitride filled cyclic olefin polymer. This material met the dual requirements for high thermal conductivity and chemical inertness.